A significant discovery within the WP MAGIC framework involves the identification of a microbial community sourced from an underground water source. This community presents a mixed presence of sulfate-utilizing and heterotrophic bacteria. To gain insights into the potential effects caused by this microbial consortium on low-pH concretes (LPCs) over the long term, a monitoring experiment in laboratory conditions has been initiated.
The experiment aims to illustrate the performance of biofilm formation on LPCs and its potential effects under both anaerobic and aerobic conditions. Additionally, the experiment will explore further conditions involving the presence of a positive ureolytic strain, discovered during the WP MAGIC framework by the Czech team.
Various techniques, including ATP assay, flow cytometry, and colony-forming unit (CFU) assessments, will be employed to validate and determine the growth of microorganisms. Scanning electron microscopy (SEM) will further confirm the formation and attachment of biofilm on the LPCs. The experiment has been set up and is scheduled to continue for three months until February 2024.